This document specifies a method for the determination of aflatoxin M₁ content in milk and milk powder. The lowest level of validation is 0,08 µg/kg for whole milk powder, i.e. 0,008 µg/l for reconstituted liquid milk. The limit of detection (LOD) is 0,05 μg/kg for milk powder and 0,005 μg/kg for liquid milk. The limit of quantification (LOQ) is 0,1 μg/kg for milk powder and 0,01 μg/kg for liquid milk.
The method is also applicable to low-fat milk, skimmed milk, low-fat milk powder and skimmed milk powder.rd edition 2021-05 Milk and milk powder — Determination of aflatoxin M content 1 — Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography Lait et lait en poudre — Détermination de la teneur en aflatoxine M — Purification par chromatographie d'immunoaffinité et 1 détermination par chromatographie en phase liquide à haute performance Reference numbers ISO 14501:2021(E) IDF 171:2021<