This document provides guidance for sample preparation, data acquisition by transmission electron microscopy, data processing, and three-dimensional image reconstruction to measure size and shape parameters of nano-objects on rod-shaped supports. The method is applicable to samples dispersed on or within an electron-transparent rod-shaped support.st edition 2021-06 Nanotechnologies — 3D image reconstruction of rod-supported nano- objects using transmission electron microscopy Nanotechnologies — Reconstruction d'images 3D de nano-objets soutenus par des tiges à l'aide de la microscopie électronique à transmission Reference number ISO/TS 22292:2021(E) ？ ISO 2021 ISO/TS 22292:2021(E) COPYRIGHT PROTECTED DOCUMENT ？ ISO