In order to solve the core issue of the energy regulation (ER) on multi-energy resource powertrain of fuel cell vehicle, the work functions of each component were defined; the mathematical algorithm model of energy regulation was established and the relevant solution was found. This algorithm was evaluated successfully on the hardware in loop (HIL) platform under three typical urban nmning cycles. The results showed ER control target had been realized and the mathematical algorithm was effective and reasonable. Based on the HIL simulation, some conclusions and ER strategies were made. According to the different power component parameters and real time control request, this algorithm should be modified and calibrated for application in the actual control system.