A series of rotenone O-alkyl oxime derivatives was designed and synthesized.Their structures were confirmed by elemental analyses,Fourier transform infrared(FTIR) and 1H NMR spectral studies,and the typical crystal structure of rotenone O-ethyl oxime(3b) was determined by X-ray diffraction.The preliminary biological activities of the new compounds were evaluated.The results of bioassays indicate that the title compounds exhibit moderate insecticidal and bactericidal activities.Among the synthesized compounds,compound 3q exhibited 90.0％ mortality against M.separata at 1000 μg/mL.Compounds 3b and 3g exhibited both 90.0％ inhibition rate against R.solani at 500 μg/mL,respectively.