A new tube-cutting method was used to measure the pressure and Mach number distribution along a microtube of 108.3 μm. Experiments were also performed concerning the average Fanning friction factors of five kinds of microtubes whose diameters range from 80.0 to 166.6 μm. It is found that the pressure distribution in a microtube becomes nonlinear at a high Mach number and the product of measured average Fanning friction factors Cf and Reynolds number Re is higher than 16. Numerical results show that the gas compressibility leads to a variation of the velocity profile from parabolic, and results in a large velocity gradient at the tube inner wall surface. The transition from laminar to turbulence in microtubes also occurs at Re≈2 300, and the phenomenon of early transition is not observed in the experiments.